Journal: Cell reports

Article Title: PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5

doi: 10.1016/j.celrep.2018.11.005

Figure Lengend Snippet:

Article Snippet: PMA , Tocris , Cat# 1201; CAS: 16561–29–8.

Techniques: Virus, Bacteria, Recombinant, Saline, Protease Inhibitor, Purification, Activation Assay, Plasmid Preparation, Software

Journal: Molecular Medicine Reports

Article Title: Protein kinase Cα stimulates hypoxia-induced pulmonary artery smooth muscle cell proliferation in rats through activating the extracellular signal-regulated kinase 1/2 pathway

doi: 10.3892/mmr.2017.7478

Figure Lengend Snippet: Expression of ERK1/2 protein in hypoxia-induced PASMC proliferation. (A) Representative western blot images and (B) quantification of ERK1/2 phosphorylation in rat PASMCs. (C) MTT assay of hypoxia-induced PAMSC proliferation following treatment with the extracellular signal-regulated kinase kinase 1/2 inhibitors PD98059 and U0126. (D) Representative western blot images and (E) quantification of ERK1/2 phosphorylation following treatment with PMA and safingol after hypoxia exposure for 72 h. Data are presented as the mean ± standard deviation*P<0.05 vs. normoxia; # P<0.05 vs. hypoxia. PASMCs, pulmonary artery smooth muscle cells; p, phosphorylated; ERK1/2, extracellular signal-regulated kinase 1/2; PMA, promoter 12-myristate 13-acetate.

Article Snippet: Prior to hypoxic treatment, cells were treated with the PKCα promoter 12-myristate 13-acetate (PMA; 0.4 mM; Alomone Labs, Israel), the PKCα special inhibitor safingol (0.6 mM; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), the extracellular signal-regulated kinase kinase (MEK1/2) inhibitor PD98059 (20 mM, Cell Signaling Technology, Inc.) or U0126 (5 mM, Cell Signaling Technology, Inc.) and incubated in a humidified atmosphere with 5% CO 2 at 37°C for 30 min one time.

Techniques: Expressing, Western Blot, MTT Assay, Standard Deviation